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KMID : 0351819930340040357
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Kyunpook University Medical Journal
1993 Volume.34 No. 4 p.357 ~ p.370
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Effect of Terminalis chebula, Sanguisorba officinalis, Rubus coreanus, and Rheum palmatum on Duck Hepatitis B Virus(DHBV) infection in Duck
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정태호
이종률/김정철/김문규/이인선/장원희/이인수/김영철/정혜리
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Abstract
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This study was performed to evaluate the effect of extracts of 4 traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to
the
HBsAg and inhibition of HBV DNA polymerase activity, We had determined that among 151 herbs, 4 herbs(Terminalis chebula, Sanguisorba officinalis, Rubus coreanus, and Rheum palmatum) showed binding capability to HBsAg which contained in the
commercial
hepatitis vaccine, at 16 g/ml of concentration and also significantly inhibited the human hepatitis B virus DNA polymerase activity.
To investigate the in vivo effect of Terminalis chebula, Sanguisorba officinalis, Rubus
coreanus, and Rheum palmatum extracts on the sequential changes of duck, each 7 DHBV carrier ducks were treated with Terminalis chebula(50mg/kg), Sanguisorba officinalis(50mg/kg), Rubus coreanus(50mg/kg), and Rheum palmatum(25mg/kg) 3 times per
week for
4 weeks.
Serum samples were obtatined before treatment and 2 and 4 weeks after administration of herbs from each group of ducks to determine the sequential chages of serum DHBV-DNA and DHBV-DNA polymerase activities. DHBV-DNA was analyzed by the method of
spot
hybridization test and the DHBV-DNA polymerase activity was determined by the method of Laplan et al. Treatment with Terminalis chebula brought sequential decrease of quantities of DHBV-DNA amount was not significant compared with the values of
before
herb administration. In the Sanguisorba officinalis treated animals, sequential changes of both the calues of DHBVA-DNA and DNA polymerase activities showed irregular fructuation. Two groups of animals treated with Rubus coreanus and Rheum
palmaium
brought the significant decrease both in the sequential examination of the serum. Terminalis chebula, Rubus coreanus, and Rheum palmatum seemed to have in vivo anti-viral effect on DHBV infected ducks.
No significant change of serum transaminase activities were determined in all animais during experimental period, and unremarkable pathological changes were noticed by the microscopic examination in the liver tissues obtained after 4 weeks.
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KEYWORD
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